SPY / Anti-SPINDLY antibody

Catalog Number: PHY1737A
Size: 150 µg
Confirmed reactivity: Arabidopsis thaliana
Database Access Number: AT3G11540

Product Information


Rabbit polyclonal antibody


SPY is a N-acetyl glucosamine transferase that may glycosylate other molecules involved in GA signaling. SPY acts as both a repressor of GA responses and as a positive regulation of cytokinin signalling. It may be involved in reducing ROS accumulation in response to stress.




Recombinant protein of SPY (834-914aa) derived from Arabidopsis thaliana AT3G11540.




150 µg


Immunogen affinity purified


Reconstitution with 150µl of 0.01 M sterile PBS.
"Note: please spin tube briefly prior to opening it to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tube".

  Stability &    

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70℃ as supplied.
6 months, -20 to -70℃ under sterile conditions after reconstitution.
1 month, 2 to 8℃ under sterile conditions after reconstitution.


The product is shipped at 4℃. Upon receipt, store it immediately at the temperature recommended above.


Application Information

  Recommended Dilution: 

Western Blot (1:1000-1:2000)
Note: Optimal dilutions/concentrations should be determined by the end user.

  Expected/apparent MW: 

101 / 96 kDa

  Confirmed Reactivity: 

Arabidopsis thaliana

  Predicted Reactivity: 

For more species homologues information, please contact tech support at tech@phytoab.com.


Application Example

6 μg nuclear protein from Arabidopsis thaliana.
Electrophoresis: 10% SDS-PAGE
Transfer: blotting to NC (nitrocellulose) membrane for 1 h.
Blocking: 5% skim milk at RT or 4℃ for 1 h.
Primary antibody: 1:2000 dilution overnight at 4℃.
Secondary antibody: 1:10000 dilution using Goat Anti-Rabbit IgG H&L (HRP) (Cat# PHY6000)
Detection: using chemiluminescence substrate and image were captured with CCD camera.