AOX1 / Anti-Plant alternative oxidase 1 antibody

Catalog Number: PHY2213A
Size: 150 µg
Confirmed reactivity: Arabidopsis thaliana
Database Access Number:AT3G22370 , AT3G22360

Product Information

Description:	Rabbit polyclonal antibody
Background:	Alternative oxidases (AOX) are quinol oxidases located in the inner mitochondrial membrane of plants. They function as terminal oxidases in the alternate electron transport pathway, oxidizing ubiquinone to reduce oxygen to water. AOX isoenzymes are including AOX1A(AT3G22370), AOX1B(AT3G22360), AOX1C(AT3G27620) and AOX1D(AT1G32350) from Arabidopsis.
Synonyms:	AOX1
Immunogen:	KLH-conjugated synthetic peptide derived from C-terminal of AOX1 protein in Arabidopsis thaliana AT3G22370, AT3G22360.
Form:	Lyophilized
Quantity:	150 µg
Purification:	Immunogen affinity purified
Reconstitution:	Reconstitution with 150µl of 0.01 M sterile PBS. "Note: please spin tube briefly prior to opening it to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tube".
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70℃ as supplied. 6 months, -20 to -70℃ under sterile conditions after reconstitution. 1 month, 2 to 8℃ under sterile conditions after reconstitution.
Shipping:	The product is shipped at 4℃. Upon receipt, store it immediately at the temperature recommended above.

Application Information

Recommended Dilution:	Western Blot (1:1000-1:2000) Note: Optimal dilutions/concentrations should be determined by the end user.
Expected/apparent MW:	40kDa(AT3G22370), 37 / 33 kDa(AT3G22360)
Confirmed Reactivity:	Arabidopsis thaliana
Predicted Reactivity:	Among 25 analyzed species, the sequence of the synthetic peptide used for immunization is 100% homologues with the sequence in Brassica napus, and 80-99% homologues with the sequence in Oryza sativa Japonica Group. For more species homologues information, please contact tech support at tech@phytoab.com.

Application Example

15 μg mitochondria protein from Arabidopsis thaliana leaf.
Electrophoresis: 15% SDS-PAGE
Transfer: blotting to NC (nitrocellulose) membrane for 1h.
Blocking: 5% skim milk at RT or 4℃ for 1h.
Primary antibody: 1:1000 dilution overnight at 4℃.
Secondary antibody: 1:10000 dilution using Goat Anti-Rabbit IgG H&L (HRP) (Cat# PHY6000)
Detection: using chemiluminescence substrate and image were captured with CCD camera.

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