叶绿体CPN20抗体

Catalog Number: PHY0378S
Size: 150 µg
Confirmed reactivity: Arabidopsis thaliana
Database Access Number: AT5G20720

Product Information

Description:	Rabbit polyclonal antibody
Background:	Co-chaperonin complex is required for substrate encapsulation during assisting the folding of the unfolded protein with the chaperonin complex. Co-chaperonin also termed as GroES, Cpn10 and Hsp10.CPN20 (AT5G20720) protein is a functional homolog of the CPN10 co-chaperonin, but it consists of two CPN10-like units joined head-to-tail by a short chain of amino acids.
Synonyms:	CPN20, ATCPN21, CHAPERONIN 20, CHCPN10, CHLOROPLAST CHAPERONIN 10, CPN10, CPN21
Immunogen:	KLH-conjugated synthetic peptide derived from N-terminal section of CPN20 protein in Arabidopsis thaliana AT5G20720.
Form:	Lyophilized
Quantity:	150 µg
Purification:	Serum Peptide affinity form antibody available upon request at info@phytoab.com.
Reconstitution:	Reconstitution with 150µl of of sterile water. "Note: please spin tube briefly prior to opening it to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tube".
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70℃ as supplied. 6 months, -20 to -70℃ under sterile conditions after reconstitution. 1 month, 2 to 8℃ under sterile conditions after reconstitution.
Shipping:	The product is shipped at 4℃. Upon receipt, store it immediately at the temperature recommended above.

Application Information

Recommended Dilution:	Western Blot (1:1000-1:2000) Note: Optimal dilutions/concentrations should be determined by the end user.
Expected/apparent MW:	27 kDa
Confirmed Reactivity:	Arabidopsis thaliana
Predicted Reactivity:	Among 25 analyzed species, the sequence of the synthetic peptide used for immunization is 100% homologues with the sequence in Medicago truncatula, Brassica napus, and 80-99% homologues with the sequence in Sorghum bicolor, Glycine max, Zea mays, Populus trichocarpa, Hordeum vulgare, Vitis vinifera, Cucumis sativus, Solanum lycopersicum, Spinacia oleracea. For more species homologues information, please contact tech support at tech@phytoab.com.

Application Example

Lane 1: Thylakoid membrane protein from Arabidopsis thaliana leaf containing 5 μg of chlorophyll.
Lane 2: 10 μl total chloroplast protein from Arabidopsis thaliana leaf.
Lane 3: 18 μg total protein from Arabidopsis thaliana leaf.
Electrophoresis: 15% SDS-PAGE
Transfer: blotting to NC (nitrocellulose) membrane for 1h.
Blocking: 5% skim milk at RT or 4℃ for 1h.
Primary antibody: 1:1000 dilution overnight at 4℃.
Secondary antibody: 1:10000 dilution using Goat Anti-Rabbit IgG H&L (HRP) (Cat# PHY6000)
Detection: using chemiluminescence substrate and image were captured with CCD camera.

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