USP7 Inhibitor Screening Assay Kit

Catalog #

79256

$820

Description

The USP7 Inhibitor Screening Assay Kit is designed to measure USP7 activity for screening and profiling applications. The USP7 Inhibitor Screening Assay Kit comes in a convenient format, with a 96-well plate, fluorogenic, ubiquitinated substrate, assay buffer, and purified USP7 enzyme for 96 enzyme reactions. USP7 is incubated with a sample containing assay buffer and Ub-AMC Substrate, and then the plate is read, measuring fluorescence using a fluorescence reader.

Species

96 rxns

UniProt #

Q93009

Applications

Useful for studying enzyme kinetics and HTS applications.

Supplied As

The USP7 Inhibitor Screening Assay Kit comes in a convenient format, with a 96-well plate, fluorogenic, ubiquitinated substrate, assay buffer, and purified USP7 enzyme for 96 enzyme reactions.

Storage/Stability

Stable at least 6 months from date of receipt, when stored as directed. Kit components require different storage conditions. Be sure to store each component at the proper temperature upon arrival.

Background

Deubiquitinases (DUBs) play important roles in pathways that are dysregulated in cancer, such as DNA repair, cell growth, and apoptosis. This makes DUBs attractive potential drug targets for many cancers and neurodegenerative diseases. Ubiquitin-specific-processing proteases (USPs) are the largest class of DUBs. USP7, also known as herpesvirus-associated ubiquitin-specific protease (HAUSP), acts as a tumor suppressor by stabilizing p53 tumor suppressor as well as a regulator of gene expression in herpes simplex virus (HSV) and Epstein-Barr virus (EBV) infections.

Synonym(s)

Ubiquitin specific peptidase 7, USP 7, HAUSP, USP-7, activity assay kit

Contraindications

DMSO >1%, strong acids or bases, ionic detergents, high salt

Format

Instructions for Use

See assay kit data sheet for detailed protocol.

References

1. Pfoh, R., et al. Deubiquitinases and the new therapeutic opportunities offered to cancer. Endocrine-Related Cancer. 2015; 22(1):T35-T54.

Warnings

Avoid freeze/thaw cycles.

Scientific Category

Deubiquitinase