50011 HDAC11, GST-tag

Catalog #50011

Size: 50 µg

Description

Human HDAC11 , full length, GenBank Accession No. NM_024827, with N-terminal GST-tag, MW= 66 kDa, expressed in baculovirus expression system.

Species

Human

Host Species

Sf9 insect cells

MW

66 kDa

Genbank #

NM_024827

Tag(s)

N-terminal GST-tag

a.a.

full length

UniProt #

Q96DB2

Applications

Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

Stability

>6 months at –80°C

Storage/Stability

>6 months at -80°C.

Shipping Temperature

-80

Synonym(s)

histone deacetylase 11, HDAC-11

Specific Activity

≥3.7 pmol/min/µg.

Assay Conditions

25 mM Tris HCl, pH 8.0, 137 mM NaCl, 2.7 mM KCl, 1 mM MgCl₂, and 0.1 mg/ml BSA, 20 µM BPS HDAC class 2a substrate (Catalog #50040), and HDAC11 (15.6 - 2000 ng). Incubation condition: 35 min at 37°C, followed by HDAC developer (Catalog #50030) for 15 min at room temperature. Fluorescence intensity is measured at ex360/em460.

Formulation

40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 16 mM glutathione, 20% glycerol

Format

Aqueous buffer solution

References

Strausberg, R.L. et al., Proc. Natl. Acad. Sci. U.S.A. 99 (26), 16899-16903 (2002).

Application Reference(s):
1. Reassessing the effects of histone deacetylase inhibitors on hippocampal memory and cognitive aging (2014)

Warnings

Avoid freeze/thaw cycles.

Scientific Category

Deacetylase

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